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KMID : 1094720230280010091
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Biotechnology and Bioprocess Engineering
2023 Volume.28 No. 1 p.91 ~ p.100
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MiR-466d Targeting MMP13 Promotes the Differentiation of Osteoblasts Exposed to a Static Magnetic Field
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Fei Tong
Hongmeng Cheng
Jun Guo
Jianyong Wu
Hongshan Ge
Zhihua Li
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Abstract
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The static magnetic field (SMF) and miRNAs are involved in the regulation of bone remodeling. Hence, this study was designed to investigate the effects of SMF on the proliferation and differentiation of osteoblasts, as well as identify related miRNAs. SMF promoted osteoblast proliferation and differentiation and inhibited osteoblast apoptosis. High-throughput sequencing identified that 41 miRNAs were upregulated and 25 miRNAs down-regulated when osteoblasts were exposed to SMF. The enrichment analysis and validation results indicated differentially expressed miR-466d and its target gene MMP13. In osteoblasts, miR-466d could directly target MMP13, and miR-466d overexpression combined with the exposure to SMF promoted differentiation, suppressing MMP13 expression. SMF increased miR-466 expression in osteoblasts, thereby inhibiting MMP13 expression and increasing osteoblast differentiation ability.
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KEYWORD
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osteoblast, proliferation and differentiation, miR-466d, MMP13, high-throughput sequencing
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